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KMID : 0861020010160020049
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Korea Journal of Herbology
2001 Volume.16 No. 2 p.49 ~ p.55
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Effect of Cibotium barometz on the Growth of Longitudinal Bone in Adolescent Male Rats
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Abstract
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In this study, we searched some herbal medicines on promoting the heights of longitudinal bone and found that the root of Cibotium barometz promotes longitudinal bone growth. The longitudinal bone growth was measured in the proximal tibia by fluorescence microscope with tetracycline. Tetracycline plays a chelate with calcium ion which is accumulated in bone forming-zones. The accumulated tetracycline made fluorescent lines corresponding to two injections. The growth rate of bones are evaluated by measuring the length between two lines. The longitudinal bone growth rate of control group for two days was 489.0±70.1 ㎛ but, C. barometz administration significantly promoted the longitudinal bone growth(100 ㎎/㎏, 764.2±41.1 ㎛, p<0.01). And the heights of proliferative and hypertrophic zones were also increased in C. barometz administered group measured by Nissl staining. To evaluate its growth promoting mechanisms, the induction of local insulin-like growth factor-1(IGF-1) and bone morphogenetic protein-2(BMP-2) were measured by using immunohistochemical method. C. barometz increased local induction of BMP-2 in hypertrophic and ossification zones, whereas IGF-1 did not increase. These results showed that administration of the extracts of C. barometz promoted longitudinal bone growth and its mechanism could be explained in part by regulation of BMP-2 induction in growth plates.
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